Game:MUSYNX

Abstract: Due to our team’s interest in finding alternate ways to treat strep throat and tonsillitis, we hope to utilize phage therapy to target colonies of Streptococcus Pyogenes in the throat and tonsils. Our ultimate goal is creating a mouthwash that contains Lactococcus lactis that would release a phage that could bind to S. Pyogenes, thus reducing its harmful effects in the cases of strep throat and tonsillitis.

Wiki Link: https://2020.igem.org/Team:ICS_BKK

Credits:
Chavis (Ta) V. - Drawer
Puripat (Plum) T. - Voice, Script Editor
Nathaphol (Khaooat) R. - Logo Designer
Kachatad (Best) P. - Subtitles
Arisa (Ing) A. - Script Editor, Video Editor
Pann S. - Script Editor

Title: Project Trojan Horse

Abstract: Our project aims to treat tonsillitis utilizing phage therapy as an alternative to antibiotics, whose drawback is resistance. Our ultimate goal is to create a mouthwash containing engineered Lactococcus lactis strains capable of releasing a phage to deactivate Streptococcus pyogenes. For computational lab, we modified over 300 strains of Phage P2, natural phage of Lactococcus lactis, to bind to S. pyogenes and achieved the highest binding affinity of -6.38 kcal/mol, compared with the original affinity of -4.50 kcal/mol between P2 and S. pyogenese. For the wet lab approach, we conducted biopanning, a screening method to identify peptides with high affinity to a given target, on Staphylococcus aureus, a common virulent gram-positive bacteria in respiratory tracts. We successfully binded one strand of Phage M13 to S. aureus. The successful binding results in both approaches lay a foundation for next year's modification of bacteriophage-containing bacteria in mouthwash delivery.

Wiki | Project Promotion