Highlight Reel from Vegas.

Awesome game last night.

Vegas GokdenKnights win 4-0 over the Pittsburgh Penguins

Watch X.com/NHL clips on https://SportsTalkSocial.com

Source: https://x.com/GoldenKnights/status/1898238780739666436

SA...

The
@BuffaloSabres go on to score SEVEN goals and pick up the Win over Pittsburgh Penguins

Source: https://x.com/NHL/status/1905422377044828452

The Pittsburgh 2020 iGEM Team presents the Bluetooth Bacteria. Inspired by Bluetooth wireless technology, the Bluetooth Bacteria is a tool that will allow wireless control of bacterial gene expression. After the attachment of magnetic nanoparticles to the surface of E. coli, an input of alternating magnetic fields will raise cell temperature and activate heat-sensitive dimers. This allows spatial and temporal control of gene expression lending itself to many biomedical and industrial applications.

https://2020.igem.org/Team:Pittsburgh

Audio by Lia Franco
Edited by Sabrina Catalano
Narration by Dara Czernikowski
Script by Angel Zheng
Subtitle by Victor So
Music by Aleksandr Shamaluev

Title: Bluetooth Bacteria

Abstract: Our ability to control the function of engineered microbes is limited within hard to reach places, such as deep within human tissue, the rhizosphere surrounding crops, or pipes in manufacturing facilities. The field of magnetogenetics has begun to consider how magnetic fields could affect biological molecules and cells. In our project, we aim to control the function of bacteria via the remote activation of an alternating magnetic field (AMF). E. coli will be engineered to respond to magnetic stimuli by attaching magnetic nanoparticles to the surface of the bacterial membrane in conjunction with cytoplasmic temperature-sensitive transcriptional repressors to regulate gene expression of the green fluorescent protein. Upon AMF stimulation, magnetic nanoparticles heat up and increase cytoplasmic temperature, thereby inducing a conformational change of the repressor dimers and allowing transcription of target genes. This technology critically couples alternating magnetic field stimulation with thermal sensitive parts to enable 'wireless' control of bacteria.

Wiki | Project Promotion

Title: ColonDetective

Description: Our project is focused on the design of a system to detect colon cancer by producing a color change in stool using engineered E coli cells.

Wiki

Title: Colon Detective: Tumor Diagnostics centered on Fimbrial Anchorage and Oxidative Stress Activation

Description: Contributing factors to high mortality rates of Colorectal Cancers (CRC) in the United States are associated with the cost and inefficiencies with colonoscopies as the initial detection method. Here we propose the development of a detection system utilizing engineered E.coli cells to produce a color change in the presence of CRC tumors and adenocarcinomas. To colonize colorectal cells, we have proposed engineering E. coli cells with chimeric fimbrial cap protein that can be anchored to surfaces. Anchoring occurs via interaction with customized antigens using a SNAP tag. We then sense a hypoxic environment in cancer cells through the oxidative stress response transcription factor OxyR. To achieve this goal, we designed and built plasmids in which OxyR promoters regulate the transcription of the B-galactosidase lac-alpha fragment. Finally, the lac-alpha released forms a complex with lac-omega resulting in functional B-galactosidase that can be used in colorimetric assays reporting OxyR mediated transcription.

Wiki